ES Cell Culture Media and Recipes
Feeder media (for 100 ml) β-mercaptoethanol Diln
10% FCS (heat inact.) 10.0 ml 7 µl β-mercaptoethanol stock
1% pen-strep (100X) 1.0 ml 10 ml sterile ddH2O
1% β-mercaptoethanol Diln 1.0 ml filter sterilize, store at 40
Dulbecco's MEM 88.0 ml DO NOT AUTOCLAVE!
100.0 ml
ES cell media (for 100 ml)
15% FCS (heat inact) 15.0 ml
1% pen-strep (100x) 1.0 ml
1% β-mercaptoethanol diln 1.0 ml
1% Non-Essent. Amino Acids (100X) 1.0 ml
1% Glutamax (100X) 1.0 ml
LIF ~0.1 ml
Dulbecco's MEM 81.0 ml
100.0 ml
2x freezing media
20% fetal bovine serum 10 ml
20% DMSO 10 ml
Dulbecco's MEM 30 ml